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MBRS PROGRAM
Home :: Lopez-Garriga

Juan López-Garriga, Ph.D.

email: SONW@caribe.net
Web page: http://sonw.uprm.edu
Current Projects with MBRS-SCORE funds:
Unraveling the Reaction between Heme (FeIV=O) Moiety and H2S: The Sulfhemoglobin Scenario

Education:

B.S. Chemistry Engineering (1976), University of Puerto Rico, Mayagüez, PR.
M.S. Chemistry (1980), University of Puerto Rico, Mayagüez, PR.
Ph. D. Physical Chemistry (1986), Michigan State University, East Lansing , MI.
Postdoctoral Physical Chemistry (1986 - 1988), Michigan State University, East Lansing , MI.
Postdoctoral Physical Chemistry (1988 - 1989), Los Alamos National Laboratory, NM.


Professional Experience:

1997-Present:
Professor, Department of Chemistry, University of Puerto Rico, Mayagüez, PR.
1992-1997:
Associate Professor, Department of Chemistry, University of Puerto Rico, Mayagüez, PR.
1989-1992:
Assistant Professor, Department of Chemistry, University of Puerto Rico, Mayagüez, PR.
1980-1981:
Research Associate, University of Puerto Rico, Mayagüez, PR.
1976-1977:
Instructor of General Chemistry, University of Puerto Rico, Arecibo, PR.

Honors and Awards:

  • Alumni Distinguish Professor, University of Puerto Rico at Mayagüez. 2001

  • Igaravídez Award in Chemistry (ACS-Puerto Rico Section). 2000

  • Presidential Awards for Excellence in Science Mathematics, and Engineering Mentoring (PAESMEM). 2000

  • University of Puerto Rico Mayagüez Scholar Award, Puerto Rico.1997, 1998

  • EPSCoR Scholarly Productivity Award, Puerto Rico . 1990, 1992, 1994, 1996, 1998

  • Camille and Henry Dreyfus Teacher-Scholar Award. 1994

  • Provost Affirmative Action Postdoctoral, Michigan State University . 1987

  • Monsanto Industrial Fellow, Michigan State University . 1986

  • Chemistry Outstanding Teaching Assistant, Michigan State University . 1984

  • Regional High School Science Fair Award, Puerto Rico , 1970.

Publications:

  • EJ. A. Gavira, W. de Jesus, A. Camara-Artigas, J. López-Garriga and J. M. García-Ruiz. "Capillary crystallization and molecular-replacement solution of haemoglobin II from the clam Lucina pectinata". Acta Crystallographica Section F: Structural Biology and Crystallization Communications. Accepted 22 January 2006.

  • De Jesus-Bonilla W, Cruz A, Lewis A, Cerda J, Bacelo DE, Cadilla CL, López-Garriga J. . "Hydrogen-bonding conformations of tyrosine B10 tailor the hemeprotein reactivity of ferryl species".. J Biol Inorg Chem. 2006 Feb 9;:1-9 [Epub ahead of print]

  • Ruth Pietri, Ruth G. León, Laurent Kiger, Michael C. Marden, Laura B. Granell, Carmen L. Cadilla and Juan López-Garriga. "Hemoglobin I from Lucina pectinata: A model for distal heme-ligand control. Biochim ". Biophys Acta. 2005 Dec 12; [Epub ahead of print]

  • Ruth Pietri, Laura Granell, Anthony Cruz, Walleska De Jesús, Ariel Lewis, Ruth Leon, Carmen L. Cadilla and Juan López Garriga. "Tyrosine B10 and heme–ligand interactions of Lucina pectinata hemoglobin II: control of heme reactivity". Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics. Volume 1747, Issue 2 , March 2005, Pages 195-203.

  • Pietri R, Granell L, Cruz A, De Jesus W, Lewis A, Leon R, Cadilla CL, Garriga JL.. "Tyrosine B10 and heme-ligand interactions of Lucina pectinata hemoglobin II: control of heme reactivity". Biochim Biophys Acta. 2005 Mar 14;1747(2):195-203. Epub 2004 Dec 19.

  • Evys Collazo, Ruth Pietri, Walleska De Jesús, Cacimar Ramos, Ana Del Toro, Ruth Gretchen León, Carmen L. Cadilla and Juan López-Garriga. "Functional Characterization of the Purified Holo Form of Hemoglobin I from Lucina pectinata Overexpressed in Escherichia coli". The Protein Journal. Volume 23, Number 4 ,May 2004, Pages: 239 - 245.

  • E. Ramirez, C. Ramos, M. Rodríguez, R. Pietri, M. Chergui and J. López-Garriga"Ultrafast studies of ferric complexes of Hemoglobin I from Lucina pectinata Femtochemistry and Femtobiology". (Ed. M.M Martin, J.T. Hynes) Elsevier N.Y. pages 395-398.

  • Jan Helbing , Luigi Bonacina, Ruth Pietri, Jens Bredenbeck, Peter Hamm, Frank van Mourik , Frédéric Chaussard , Alejandro Gonzalez-Gonzalez , Majed Chergui, Cacimar Ramos-Alvarez , Carlos Ruiz and Juan López-Garriga. "Time-Resolved Visible and Infrared Study of the Cyano Complexes of Myoglobin and of Hemoglobin I from Lucina pectinata". Biophysical Journal 87:1881-1891 (2004).

  • Ruth Gretchen León, Hélène Munier-Lehmann, Octavian Barzu, Véronique Baudin-Creuza, Ruth Pietri, Juan López-Garriga and Carmen L. Cadilla. "High-level production of recombinant sulfide-reactive hemoglobin I from Lucina pectinata in Escherichia coli. High yields of fully functional holoprotein synthesis in the BLi5 E. coli strain". Protein Expression and Purification Volume 38, Issue 2 , December 2004, Pages 184-195.

  • De Jesús W, Ramírez E, Cerda J, López-Garriga, J Evidence for a Nonhydrogen Bonded Compound II in the Cyclic Reaction of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide” Biospectroscopy 2001 (accepted for publication).

  • RosadoT., Antommattei F, Cadilla C, López-Garriga J .Expression and Purification of Recombinant Hemoglobin I from Lucina pectinata” , J. Protein. Chem. 20 , 311-315, 2001

  • De Jesus W, Cortes J, López-Garriga J . Formation of Compounds I and Compound II Ferryl Species in the Reactions of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide. Arch. Biochem. Biophys., 390, 304-308, 2001.

  • Oren A , López-Garriga J , Montalvo R , Vreeland R H, Ventosa A , Kamekura M . Natrinema thermotolerans sp. nov., a halophilic archaeon from Puerto Rico . Inter. J. Syst. and Evol. Microbiol 50:1065-1071, 2000.

  • Antommattei FM, Rosado T , Cadilla CL, López-Garriga J . The cDNA-Derived Amino Acid sequence of HbI from Lucina pectinata. J Protein Chem 18:831-836, 1999.

  • Cerda J, Echevarria Y, López-Garriga J . Resonance Raman Studies of the Heme-Ligand Active Site of Hemoglobin I from Lucina pectinata. Biospectroscopy 5:289-301, 1999.

  • Montalvo R, Vreeland RH, Oren A, Kessel M, Betancourt C, López-Garriga J . Halogeometricum borinquense gen. nov., sp. Nov., a Novel Halophilic Archaeon from Puerto Rico. Inter J Syst. Bacteriol 48:1305-1312, 1998.

  • Silfa E, Almeida M, Cerda J F, Wu S, López-Garriga J . Orientation of the Heme Vinyl Groups in the Hydrogen Sulfide-Binding Hemoglobin I from Lucina pectinata. Biospectroscopy 4:311-326 , 1998.

  • Montalvo R, Ruiz A, López-Garriga J. New Record of Extremely Halophilic Archaebacteria for Puerto Rico and the Caribbean . Caribbean J. Science 33: 98-104 , 1997.

  • Navarro A, Maldonado M, Gonzalez-Lagoa J, López-Garriga J , Colon J. Control of CO Binding States and Dynamics in HbI of Lucina pectinata by Aromatic Residues. Inorg Chim Acta 243:161-166, 1996.

  • Oertling WA, Wu W, López-Garriga J , Kim Y, Chang CK. Resonance Raman Characterization of Free_Base Porphycene and Ni(II), Co(II), Fe(II) and Fe(III) Porphycene Complexes. J Am Chem Soc 113:127-134, 1991.

  • López-Garriga J , Oertling WA, Kean RT, Hoogland H, Wever R, Babcock GT. Metal_Ligand Vibrations of Cyanoferric Myeloperoxidase and Cyanoferric Horseradish Peroxidase: Evidence for a Constrained Heme Pocket for Myeloperoxidase. Biochemistry 29:9387-9395, 1990.

  • Dyer RB, López-Garriga J, Einarsdóttir O, Woodruff WH. The Orientation of CO in Carbonmonoxy Cytochrome Oxidase and Its Transient Photoproducts. Direct Evidence from Time_Resolved Infrared Linear Dichroism. J Am Chem Soc 111:8962-8963, 1989



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