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NF-IL6: A Human Transcription Factor Implicated in Monocyte Differentiation

Elsie L. Parés-Matos Ph.D.


Monocytes play a central role in the immune system and their development passes through several step until become macrophages. During the monocyte/macrophage differentiation, external differentiating signals regulate the expression set of transcription factors. In particular, the CCAAT enhancer-binding proteins (C/EBP) have a critical role in this process.

Expression of C/EBPα gradually decreases with macrophage differentiation, whereas expression of C/EBPβ (also known as NF-IL6) and C/EBPδ is up-regulated during macrophage maturation. Because the importance of NF-IL6 in monocytes/macrophages differentiation and its importance in inflammatory responses, some studies have aimed at identifying the transcription factors that regulate the expression of NF-IL6 gene in various cell-types and organism. However, these studies do not explain the responsiveness of human NF-IL6 in two human leukemic models of myelomonocytic development HL-60 and THP-1. The research design described is aimed to reveal changes and/or similarities between all three lines (U937, HL-60 and THP-1) in terms of the responsiveness of the proximal promoter of the human NF-IL6 gene to LPS and PMA.

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