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Identification of Bacillus Anthracis Lethal Factor specific inhibitors peptides using T7 Phage Display

Researcher
Carlos Ríos-Velázquez Ph.D

Abstract

The long term goal of this research is to identify and map new potential interacting partners to toxin using phage display technology. The knowledge obtained by these interaction ligands will allow understanding on molecular pathogenesis networking (signal transduction), and the development of biomarkers and inhibitors for molecular therapeutics.

The proposed research seeks to identify toxin specific interacting peptides inhibitors, using the anthrax toxin component lethal factor (LF) as target protein (TP). The absorbed TP will be exposed to pre-made human cDNA phage display libraries. The advantages of using the phage displaying systems are that this technology allows the use of high number and complex (combinatorial) sequences to be displayed in the libraries, and its rapid screening process. We plan to use several peptide display systems such as M13 and T7.

The peptide display libraries will be screened by rounds of affinity- selection (biopannings), and the isolated candidates will be assayed for specificity. The target interacting peptides will be sequenced and screened in genome data-bases for proteins (toxin specific partners) with consensus regions similar to target specific peptides. The confirmed novel interacting partners will be used to propose their molecular role in the toxin pathogenesis.




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