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Viability of Single Cells Flowing through Microfluidic Channels Designed for Bioelectronic Applications

Researcher
Rubén E. Díaz-Rivera Ph.D

Abstract

Microfluidic technology is changing the way researchers study and manipulate complex biological systems. One of the techniques that have been greatly enhanced by microfluidics is single-cell electroporation.
Single-cell electroporation offers a way to control the mass transport across the plasma membrane of cells, but at a cost, low throughput. In order to use this promising technique in real biomedical applications such as ex-vivo genetic immunotherapy and controlled molecular transport in primary cells, its throughput needs to be improved.
It has been shown that it is possible to fabricate a device that provides controlled electroporation and high-throughput by using microfluidic technology. However, the behavior and health of biological cells when placed in the artificial fluidic environment is unknown. Therefore, the objective of this research proposal is to investigate the possibility of electroporating single cells in a safe fashion when using the proposed microfluidic device by monitoring the cell’s health.




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